Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells (NPCs)

9.9K Views

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10:47 min

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March 2nd, 2018

DOI :

10.3791/56628-v

March 2nd, 2018

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Madeline Williams*¹, Smrithi Prem*¹, Xiaofeng Zhou¹, Paul Matteson², Percy Luk Yeung³, Chi-Wei Lu³, Zhiping Pang⁴, Linda Brzustowicz⁵, James H. Millonig², Emanuel Dicicco-Bloom¹

¹Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School, ²Center for Advanced Biotechnology and Medicine, Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School, ³The Child Health Institute of NJ, Department of Obstetrics, Gynecology, and Reproductive Services, Rutgers Robert Wood Johnson Medical School, ⁴The Child Health Institute of NJ, Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School, ⁵Department of Genetics, Rutgers University

Summary

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Keywords Neural Precursor Cells

Neurodevelopmental Phenotypes

Neural Development

Cell Characterization

Neurodevelopmental Disorders

Neuropsychiatric Disorders

Rapid And Reproducible Method

Chapters in this video

0:04

Title

1:08

Collecting NPCs for Plating of Experimental Conditions

2:30

Neural Precursor Cell DNA Synthesis Assay

3:47

S Phase Entry Assay

4:39

Neural Precursor Cell Number Assay

5:41

Neural Precursor Cell Neurosphere Migration Assay

7:58

Results: Use of Phase-contrast and Fluorescence Microscopy to Study the Proliferation, Migration, and Neurite Outgrowth of Neural Precursor Cells

9:51

Conclusion

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