Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction

0:45

Immobilization of the Receptor (Integrin α2A-domain) to a Microtiter Plate

2:09

Preparation of a Serial Dilution Row of the Ligand (Rhodocetin)

3:25

Binding of Ligand at Different Concentrations to Immobilized Receptor

5:03

Quantification of Receptor-bound Ligand by ELISA

7:06

Evaluation of the Titration Signals

9:30

Results: Rhodocetin Binds with Lower Affinity to Mutant Integrin α2A-domain

10:58

Conclusion