Preparation of Agar Pad, Preparation of Bacteria-amoeba Sample, and Image Acquisition
4:05
Results: Virulence of P. aeruginosa is Upregulated in Surface-attached Populations
4:38
Conclusion
Transkript
This method can help answer key questions in the bacterial pathogenesis field, such as when are host-killing virulence effectors expressed in bacterial cells, and what are the virulence states of surface-attached implantonic cells? The main advant
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Here, we present a protocol to measure the virulence of planktonic or surface-attached bacteria using D. discoideum (amoeba) as a host. Virulence is measured over a period of 1 h and host killing is quantified using fluorescence microscopy and image analysis. We demonstrate this protocol using the bacterium P. aeruginosa.