Distance from the Center of the Lens Nucleus to the Anterior Pole Measurement (a - r)
5:10
Results: Representative In Vitro and In Vivo Larval Zebrafish Lens Morphology Comparison
6:36
Conclusion
Transkript
Our protocols provide a starting point for functional studies of lens development and physiology and for reverse genetic screening of lens phenotypes in zebrafish. Our protocols bridge in vitro and in vivo analyses of zebrafish lens membrane morph
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These protocols were developed to analyze cortical lens morphology, structural integrity of the zebrafish lens sutures in fixed and live lenses and to measure the position of the zebrafish lens nucleus along the anterior-posterior axis.