Results: Identification and Labeling Results of Metabolites
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Conclusion
Transkript
Cell-free protein synthesis is an emerging technology in systems and synthetic biology for the in vitro production of proteins. Cell-free protein synthesis systems have no cell wall, thus we have direct access to metabolites. In this protocol, we
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Here, we present a robust protocol to quantify 40 compounds involved in central carbon and energy metabolism in cell-free protein synthesis reactions. The cell-free synthesis mixture is derivatized with aniline for effective separation using reversed-phase liquid chromatography and then quantified by mass spectrometry using isotopically labelled internal standards.