Fabricating and Assembly of the Acoustofluidic Microdevice
3:02
Sample Preparation
5:41
Measuring the Compressibility of Cell and Nucleus
7:29
Results: Measuring Cell or Nucleus Compressibility Based on Acoustofluidic Microdevice
8:38
Conclusion
Transkript
Cell mechanics play an important role in tumor metastasis, malignant transformation of cells, and radiosensitivity. Acoustofluidic method can realize fast and non-destructive measurement in suspended states. This technique can be used for mirrorin
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Here a protocol is presented to build a fast and non-destructive system for measuring cell or nucleus compressibility based on acoustofluidic microdevice. Changes in mechanical properties of tumor cells after epithelial-mesenchymal transition or ionizing radiation were investigated, demonstrating the application prospect of this method in scientific research and clinical practice.