<meta charset="utf8"></head><body>Azaltılmış doku hasarı için optimize edilmiş sıçan intracameral enjeksiyon tekniği

<ol>
	<li>Ramsay, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Corneal+and+conjunctival+drug+permeability:+Systematic+comparison+and+pharmacokinetic+impact+in+the+eye.">Corneal and conjunctival drug permeability: Systematic comparison and pharmacokinetic impact in the eye.</a> Eur J Pharm Sci. 119, 83-89 (2018).</li><li>Cholkar, K., Dasari, S. R., Pal, D., Mitra, A. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Eye: Anatomy, Physiology and Barriers to Drug Delivery. Ocular Transporters and Receptors. , (2013).</li><li>Prausnitz, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Permeability+of+cornea,+sclera,+and+conjunctiva:+A+literature+analysis+for+drug+delivery+to+the+eye.">Permeability of cornea, sclera, and conjunctiva: A literature analysis for drug delivery to the eye.</a> J Pharm Sci. 87 (12), 1479-1488 (1998).</li><li>Varela-Fern&#225;ndez, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Drug+delivery+to+the+posterior+segment+of+the+Eye:+Biopharmaceutic+and+pharmacokinetic+considerations.">Drug delivery to the posterior segment of the Eye: Biopharmaceutic and pharmacokinetic considerations.</a> Pharmaceutics. 12 (3), 269 (2020).</li><li>Gautam, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intracameral+drug+delivery:+A+Review+of+agents,+indications,+and+outcomes.">Intracameral drug delivery: A Review of agents, indications, and outcomes.</a> J Ocul Pharmacol Ther. 39 (2), 102-116 (2023).</li><li>Shah, T. J., Conway, M. D., Peyman, G. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intracameral+dexamethasone+injection+in+the+treatment+of+cataract+surgery+induced+inflammation:+design,+development,+and+place+in+therapy.">Intracameral dexamethasone injection in the treatment of cataract surgery induced inflammation: design, development, and place in therapy.</a> Clin Ophthalmol. 12, 2223-2235 (2018).</li><li>Perlman, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+models+of+human+disease:+An+evolutionary+perspective.">Mouse models of human disease: An evolutionary perspective.</a> Evol Med Public Health. 2016 (1), 170-176 (2016).</li><li>Chawla, S., Jena, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> The Anatomy and Physiology of Laboratory Rat.Essentials of Laboratory Animal Science: Principles and Practices. , (2021).</li><li>Lundstr&#246;m, M., Wejde, G., Stenevi, U., Thorburn, W., Montan, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endophthalmitis+after+cataract+surgery:+a+nationwide+prospective+study+evaluating+incidence+in+relation+to+incision+type+and+location.">Endophthalmitis after cataract surgery: a nationwide prospective study evaluating incidence in relation to incision type and location.</a> Ophthalmology. 114 (5), 866-870 (2007).</li><li>Fine, I. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Clear+corneal+incisions.">Clear corneal incisions.</a> Int Ophthalmol Clin. 34 (2), 59-72 (1994).</li><li>Herretes, S., Stark, W. J., Pirouzmanesh, A., Reyes, J. M. G., McDonnell, P. J., Behrens, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inflow+of+ocular+surface+fluid+into+the+anterior+chamber+after+phacoemulsification+through+sutureless+corneal+cataract+wounds.">Inflow of ocular surface fluid into the anterior chamber after phacoemulsification through sutureless corneal cataract wounds.</a> Am J Ophthalmol. 140 (4), 737-740 (2005).</li><li>Masket, S., Belani, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Proper+wound+construction+to+prevent+short-term+ocular+hypotony+after+clear+corneal+incision+cataract+surgery.">Proper wound construction to prevent short-term ocular hypotony after clear corneal incision cataract surgery.</a> J Cataract Refract Surg. 33 (3), 383-386 (2007).</li><li>Taban, M., Rao, B., Reznik, J., Zhang, J., Chen, Z., McDonnell, P. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dynamic+morphology+of+sutureless+cataract+wounds+-+Effect+of+incision+angle+and+location.">Dynamic morphology of sutureless cataract wounds - Effect of incision angle and location.</a> Surv Ophthalmol. 49, S62-S72 (2004).</li><li>Belforte, N., Sande, P. H., de Zaval&#237;a, N., Dorfman, D., Rosenstein, R. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Therapeutic+benefit+of+radial+optic+neurotomy+in+a+rat+model+of+glaucoma.">Therapeutic benefit of radial optic neurotomy in a rat model of glaucoma.</a> PLoS One. 7 (3), e34574 (2012).</li><li>Moreno, M. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+new+experimental+model+of+glaucoma+in+rats+through+intracameral+injections+of+hyaluronic+acid.">A new experimental model of glaucoma in rats through intracameral injections of hyaluronic acid.</a> Exp Eye Res. 81 (1), 71-80 (2005).</li><li>Belforte, N., Sande, P., de Zaval&#237;a, N., Knepper, P., Rosenstein, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+chondroitin+sulfate+on+intraocular+pressure+in+rats.">Effect of chondroitin sulfate on intraocular pressure in rats.</a> Invest Ophthalmol Vis Sci. 51 (11), 5768-5775 (2010).</li><li>Matsumoto, Y., Kanamori, A., Nakamura, M., Negi, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rat+chronic+glaucoma+model+induced+by+intracameral+injection+of+microbeads+suspended+in+sodium+sulfate-sodium+hyaluronate.">Rat chronic glaucoma model induced by intracameral injection of microbeads suspended in sodium sulfate-sodium hyaluronate.</a> Jpn J Ophthalmol. 58 (3), 290-297 (2014).</li><li>Liu, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+rat+model+of+ocular+hypertension+by+a+single+intracameral+injection+of+cross-linked+hyaluronic+acid+hydrogel+(Healaflow&#174;+).">A novel rat model of ocular hypertension by a single intracameral injection of cross-linked hyaluronic acid hydrogel (Healaflow&#174; ).</a> Basic Clin Pharmacol Toxicol. 127 (5), 361-370 (2020).</li><li>Bowen, R. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparative+analysis+of+the+safety+and+efficacy+of+intracameral+cefuroxime,+moxifloxacin+and+vancomycin+at+the+end+of+cataract+surgery:+a+meta-analysis.">Comparative analysis of the safety and efficacy of intracameral cefuroxime, moxifloxacin and vancomycin at the end of cataract surgery: a meta-analysis.</a> Br J Ophthalmol. 102 (9), 1268-1276 (2018).</li><li>Kato, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prophylactic+antibiotics+for+postcataract+surgery+endophthalmitis:+a+systematic+review+and+network+meta-analysis+of+6.8+million+eyes.">Prophylactic antibiotics for postcataract surgery endophthalmitis: a systematic review and network meta-analysis of 6.8 million eyes.</a> Sci Rep. 12 (1), 17416 (2022).</li><li>Wang, M., Liu, Y., Dong, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+cefuroxime+intracameral+injection+antibiotic+prophylactic+on+postoperative+endophthalmitis+wound+post-cataract:+A+meta-analysis.">Effect of cefuroxime intracameral injection antibiotic prophylactic on postoperative endophthalmitis wound post-cataract: A meta-analysis.</a> Int Wound J. 20 (5), 1376-1383 (2023).</li><li>Katz, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intracameral+cefuroxime+and+the+incidence+of+post-cataract+endophthalmitis:+an+Israeli+experience.">Intracameral cefuroxime and the incidence of post-cataract endophthalmitis: an Israeli experience.</a> Graefes Arch Clin Exp Ophthalmol. 253 (10), 1729-1733 (2015).</li><li>Lipnitzki, I., Ben Eliahu, S., Marcovitz, A. L., Ezov, N., Kleinmann, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intraocular+concentration+of+moxifloxacin+after+intracameral+injection+combined+with+presoaked+intraocular+lenses.">Intraocular concentration of moxifloxacin after intracameral injection combined with presoaked intraocular lenses.</a> J Cataract Refract Surg. 40 (4), 639-643 (2014).</li><li>Colleaux, K. M., Hamilton, W. K., Morgan, R. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+prophylactic+antibiotics+and+incision+type+on+the+incidence+of+endophthalmitis+after+cataract+surgery.">Effect of prophylactic antibiotics and incision type on the incidence of endophthalmitis after cataract surgery.</a> Can J Ophthalmol. 35 (7), 373-378 (2000).</li><li>Libre, P. E., Della-Latta, P., Chin, N. X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intracameral+antibiotic+agents+for+endophthalmitis+prophylaxis:+A+pharmacokinetic+model.">Intracameral antibiotic agents for endophthalmitis prophylaxis: A pharmacokinetic model.</a> J Cataract Refract Surg. 29 (9), 1791-1794 (2003).</li><li>Carino, N. S., Slomovic, A. R., Chung, F., Marcovich, A. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Topical+tetracaine+versus+topical+tetracaine+plus+intracameral+lidocaine+for+cataract+surgery.">Topical tetracaine versus topical tetracaine plus intracameral lidocaine for cataract surgery.</a> J Cataract Refract Surg. 24 (12), 1602-1608 (1998).</li><li>Minakaran, N., Ezra, D. G., Allan, B. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Topical+anaesthesia+plus+intracameral+lidocaine+versus+topical+anaesthesia+alone+for+phacoemulsification+cataract+surgery+in+adults.">Topical anaesthesia plus intracameral lidocaine versus topical anaesthesia alone for phacoemulsification cataract surgery in adults.</a> Cochrane Database Syst Rev. 7 (7), (2020).</li><li>Dan-Ni, W., Li-Dian, H., Zhi-Guo, P., Qiang, W., Lei, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intracameral+anti-VEGF+injection+for+advanced+neovascular+glaucoma+after+vitrectomy+with+silicone+oil+tamponade.">Intracameral anti-VEGF injection for advanced neovascular glaucoma after vitrectomy with silicone oil tamponade.</a> Int J Ophthalmol. 14 (3), 129-135 (2021).</li><li>Bhagat, P. R., Agrawal, K. U., Tandel, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Study+of+the+effect+of+injection+bevacizumab+through+various+routes+in+neovascular+glaucoma.">Study of the effect of injection bevacizumab through various routes in neovascular glaucoma.</a> J Curr Glaucoma Pract. 10 (2), 39-48 (2016).</li><li>Al-Qaysi, Z. K., Beadham, I. G., Schwikkard, S. L., Bear, J. C., Al-Kinani, A. A., Alany, R. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sustained+release+ocular+drug+delivery+systems+for+glaucoma+therapy.">Sustained release ocular drug delivery systems for glaucoma therapy.</a> Expert Opin Drug Deliv. 20 (7), 905-919 (2023).</li><li>Eghrari, A. O., Gottsch, J. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fuchs'+corneal+dystrophy.">Fuchs' corneal dystrophy.</a> Expert Rev Ophthalmol. 5 (2), 147-159 (2010).</li></ol>

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Alizarin Red&#160;</td>
			<td>Alpha Aesar</td>
			<td>042040.5</td>
			<td></td>
		</tr>
		<tr>
			<td>Buprenorphine&#160;</td>
			<td>Richter pharma</td>
			<td>102047</td>
			<td></td>
		</tr>
		<tr>
			<td>Dexamethasone 0.1%&#160;</td>
			<td>Fisher Pharmaceutical</td>
			<td>393102-0413</td>
			<td></td>
		</tr>
		<tr>
			<td>Hamilton glass syringe 10 &#956;L&#160;</td>
			<td>Hamilton Co.</td>
			<td>721711</td>
			<td></td>
		</tr>
		<tr>
			<td>Hoeschst</td>
			<td>Merck</td>
			<td>B2261</td>
			<td></td>
		</tr>
		<tr>
			<td>Ketamine</td>
			<td>Bremer pharma GMBH (medimarket)</td>
			<td>17889</td>
			<td></td>
		</tr>
		<tr>
			<td>Ofloxacin 0.3% eye drops</td>
			<td>Allergan</td>
			<td>E92170</td>
			<td></td>
		</tr>
		<tr>
			<td>Oxybuprocaine Hydrochloride 0.4%&#160;</td>
			<td>Fisher Pharmaceutical</td>
			<td>N/A</td>
			<td></td>
		</tr>
		<tr>
			<td>Pentobarbital sodium 200 mg/mL</td>
			<td>CTS</td>
			<td>N/A</td>
			<td></td>
		</tr>
		<tr>
			<td>Slit microscope&#160;</td>
			<td>Haag-streit bern</td>
			<td>b-90019115</td>
			<td></td>
		</tr>
		<tr>
			<td>Sprague-Dawley Rats</td>
			<td>Envigo</td>
			<td>N/A</td>
			<td></td>
		</tr>
		<tr>
			<td>Stiletto blade 31 G 0.8 mm&#160;</td>
			<td>Tecfen medical (skymed)</td>
			<td>QKN2808</td>
			<td></td>
		</tr>
		<tr>
			<td>Surgical microscope</td>
			<td>Zeiss</td>
			<td>OPMI-6 CFC</td>
			<td></td>
		</tr>
		<tr>
			<td>Trypan Blue</td>
			<td>Sartorius</td>
			<td>03-102-1B</td>
			<td></td>
		</tr>
		<tr>
			<td>Xylazine</td>
			<td>Eurovet Animal Health&#160;</td>
			<td>615648</td>
			<td></td>
		</tr>
	</tbody>
</table>

intracameral injection in rats with low risk of adverse effects

The bioavailability of compounds delivered by topical administration to the surface of the eye is greatly limited, typically &#60;5%1. Compounds administered by eye drops are mainly&#160;eliminated by drainage, induced lacrimation, tear fluid turnover, and conjunctival absorption. In addition, the permeation of compounds through the ocular surface is highly restricted by the cornea-conjunctiva barrier1,2,3. The cornea is composed of three main layers: the outermost epithelium, the intermediate stroma, and the innermost endothelium. The superficial corneal epithelium is interconnected by strong&#160;tight junctions and creates high paracellular resistance, which is the main barrier to substance permeability. Multiple epithelium layers further limit the permeation of hydrophilic and large molecules through the intercellular spaces of the cornea epithelium. Succeeding the epithelium, the stroma is composed of collagen fibers and contains aqueous pores. In contrast to the corneal epithelium, the stroma allows the movement of hydrophilic drugs; however, it is greatly impermeable to lipophilic compounds1,2,3. Together, the corneal epithelium and stromal layers present major tissue barriers that limit drug absorption. The corneal endothelium is not considered to restrict drug transport.
Alternative to the corneal delivery route is the conjunctival route. The conjunctiva is a multi-epithelium layer that covers the inner side of the eyelids and the anterior part of the sclera. The conjunctiva is characterized by fewer tight junctions than the corneal epithelium, allowing better permeability of hydrophilic drugs. However, vascularization of the conjunctiva results in systemic absorption of a large fraction of the administered molecules, again greatly limiting the bioavailability of delivered compounds to the anterior chamber1,2. An efficient way to bypass the outer ocular permeability barriers is to deliver the drug directly into the region of interest. For example, intravitreal injection is common for delivery into the vitreous humor4. Likewise, intracameral injection is utilized for delivery into the anterior chamber5. Establishing an efficient concentration at the anterior chamber is critical to various clinical situations, such as the treatment of infection by intracameral injection of antibiotics and postoperative anti-inflammatory treatments in cataract surgeries. Despite the advantage of improved substance bioavailability granted by intracameral injection, there are major safety concerns that should be considered. For example, intracameral drug injection may induce increased intraocular pressure, toxic anterior segment syndrome, and toxic endothelial cell destruction syndrome5,6. It is, therefore, essential to carefully assess in pre-clinical studies the efficacy and safety of drugs delivered by intracameral injections to maximize treatment efficiency and minimize potential adverse effects in patients.
Experimental animal models are indispensable in pre-clinical studies to investigate new treatments. Small rodents, such as mice and rats, are the most commonly utilized laboratory animals for such purposes. These animals exhibit numerous similarities to human anatomy and physiology, providing valuable insights. Moreover, their use is economically advantageous due to their small size, ease of maintenance, fast gestation, and ability to produce large numbers of offspring7.
Despite the widespread use of small rodents in eye disease models, their unique eye dimensions and anatomy pose significant challenges during experimental manipulations. For instance, procedures like intracameral injections, which are relatively straightforward in humans, become technically demanding in mice and rats. The challenges stem from factors such as the small volume of aqueous humor, the relatively large and inflexible lens, and the obstructive positioning and curvature of the lens within the rodents&#39; eyes (Figure 1)8. These challenges increase the risk of damage during intracameral injections in rodents, leading to potential adverse effects and introducing experimental variability that can impact the validity of study conclusions. In our research, we have successfully developed a procedure for safe intracameral injection in rats. The technique involves creating a long, flat, self-sealing tunnel in the cornea into the anterior chamber. This method not only ensures precision but also enhances experimental reproducibility, addressing the issues associated with injection techniques in small rodents.
<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/66662/66662fig01.jpg" /> 
Figure 1: Schematic representation of the anatomical anterior segment features of rat and human eyes. <a href="https://www.jove.com/files/ftp_upload/66662/66662fig01large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>

<meta charset="utf8"></head><body>Yazar Spotlight: Kemirgen Oftalmolojisinde Hassasiyeti Artırmak için İntrakameral Enjeksiyonlar için Yeni Bir Protokol

Yan Etki Riski Düşük Olan Sıçanlarda İntrakameral Enjeksiyon

Intracameral injections allow to bypass the outer ocular barrier and directly deliver compounds to the aqueous humor. However, conducting intracameral injection in rodent present technical challenges that may result in adverse effect and impact the experimental outcome. We, therefore, design the protocol considering the red-eye anatomy to allow intracameral injection with minimal risk of adverse effects.Intracameral injections in rodents involve the risk of tissue damage due to the small eye dimensions, limited aqueous humor of volume, and lens position. Tissue damage during manipulation can lead to complication, like anterior chamber shallowing, lens injury, endothelial cell damage, and inflammation. All of this cause experimental variability and influence findings.The length and angle of the incision are critical. We make the incision in the central corneal area where the anterior chamber is the deepest at an angle close to flat. This generates a long tunnel, which helps in reducing the loss of aqueous humor.Injecting through the tunnel improves precision and reduces the likelihood of touching the lens. The described intracameral injection method can be utilized in various experimental settings to generate disease models or assess different treatments. We expect that the precision and reproducibility of this injection technique will be a valuable tool in experimental ophthalmology.

Intracameral injection is a standard administration routine in ophthalmology. The application of intracameral injection in rodents for research is ...

intracameral-injection-in-rats-with-low-risk-of-adverse-effects

Research

JoVE Journal

Biology

Intracameral Injection in Rats with Low Risk of Adverse Effects

417 Görüntüleme.  Kaplan Medical Center. İntrakameral enjeksiyonlar, dış oküler bariyeri atlamaya ve bileşikleri doğrudan sulu mizaha iletmeye izin verir. Bununla birlikte, kemirgenlerde intrakameral enjeksiyonun yapılması, olumsuz etkiye neden olabilecek ve deneysel sonucu etkileyebilecek teknik zorluklar sunar. Bu nedenle, kırmızı göz anatomisini göz önünde bulundurarak protokolü, minimum yan etki riski ile intrakameral enjeksiyona izin verecek şekilde tasarlıyoruz.Kemirge...