0:00
Overview
1:11
Principles of Gram Staining
2:52
Sample Collection and Isolation
4:27
Preparation of Bacterial Smears
5:26
Gram Staining and Visualization
7:16
Applications
9:07
Summary
Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona
Demonstrating Author: Luisa Ikner
The spectrum of research in environmental microbiology is broad in scope and application potential. Whether the work is bench-scale with known bacterial isolates, or in the field collecting soil or water samples containing unknown bacterial isolates, the ability to quickly and visually discern culturable populations of interest remains of great import to environmental microbiologists even today with the abundance of molecular techniques available for use. This video will demonstrate one such technique, known as Gram staining.
1. Sample Collection
The Gram stain is used in the many sub-fields of both environmental and clinical microbiology. Water quality scientists may use the Gram stain as a confirmatory tool for the detection of fecal bacteria in water samples. Bacterial isolates from soils are Gram stained in order to further characterize culturable soil communities. For environmental microbiologists, Gram stain aids in the categorization of bacterial populations according to cell wall structure. This, in turn, provides information about the general ability of
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