Single Molecule Methods for Monitoring Changes in Bilayer Elastic Properties

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12:20 min

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November 3rd, 2008

DOI :

November 3rd, 2008

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Helgi Ingolfson¹, Ruchi Kapoor², Shemille A. Collingwood², Olaf Sparre Andersen²

¹Department of Physiology and Biophysics, Weill Cornell Medical College, ²Department of Physiology and Biophysics, Weill Cornell Medical College of Cornell University

Summary

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Single Molecule Methods

Bilayer Elastic Properties

Membrane Protein Function

Cell Membrane Lipid Composition

Lipid protein Interactions

Lipid Bilayer protein Interactions

Pharmacological Research

Pharmaceuticals

Lipid Bilayer Material Properties

Altered Membrane Protein Function

Gramicidin Channels

Conformational Changes

Gramicidin Subunits

Properties Of The Lipid

Gramicidin Channel Current

Reporter Compounds

Chapters in this video

0:00

Title

0:12

Introduction

1:18

Preparing for Bilayer Experiments

3:44

Forming a Membrane

4:43

Gramicidin Channels

5:43

Recording the Control Current Traces

6:38

Recording the Experimental Current Traces

7:41

Data Analysis

11:54

Conclusion

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