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12:20 min
November 3rd, 2008
DOI :
10.3791/1032-v
章节
0:00
Title
0:12
Introduction
1:18
Preparing for Bilayer Experiments
3:44
Forming a Membrane
4:43
Gramicidin Channels
5:43
Recording the Control Current Traces
6:38
Recording the Experimental Current Traces
7:41
Data Analysis
11:54
Conclusion
摘要
Membrane protein function is regulated by the cell membrane lipid composition. This video-article details how to form a patch using bilayer patch electrodes, as well as how to use gramicidin channels as reporters of altered membrane properties.
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