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Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence (TIRF) Microscopy

DOI :

10.3791/64970-v

8:55 min

February 17th, 2023

February 17th, 2023

1,963 Views

1Biophysics Department, Institute of Biomaterials and Biomolecular Systems, University of Stuttgart

This protocol describes how to use TIRF microscopy to track individual ion channels and determine their activity in supported lipid membranes, thereby defining the interplay between lateral membrane movement and channel function. It describes how to prepare the membranes, record the data, and analyze the results.

Tags

Single molecule Imaging

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