Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy

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07:11 min

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April 1st, 2009

DOI :

April 1st, 2009

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Ellie Graeden¹^,², Hazel Sive¹^,²

¹Department of Biology, MIT - Massachusetts Institute of Technology, ²Whitehead Institute for Biomedical Research, MIT - Massachusetts Institute of Technology

Summary

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Zebrafish Embryonic Brain

Confocal Microscopy

Cell Shape Changes

Cell Rearrangements

Zebrafish Brain Development

Neural Tube Morphogenesis

Vertebrate Brain Structure

Membrane targeted Green Fluorescent Protein memGFP

Agarose Mounting

Fluorescent Imaging

Chapters in this video

0:00

Title

0:37

Introduction

1:09

Preparing for Injection

2:14

Injecting mRNA

4:12

Mounting and Imaging Embryos

6:14

Representative Results: Confocal Imaging

6:35

Conclusion

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