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4-Dimensional Imaging of Zebrafish Optic Cup Morphogenesis

DOI :

10.3791/62155-v

7:26 min

May 26th, 2021

May 26th, 2021

3,078 Views

1Department of Human Genetics, University of Utah

This protocol describes an approach for in toto labeling and multidimensional imaging of zebrafish early eye development. We describe labeling, embedding, and four dimensional (4D) imaging using laser scanning confocal microscopy, and considerations for optimizing acquisition of datasets for dissecting mechanisms of optic cup morphogenesis.

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4 Dimensional Imaging

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