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Visualizing the Developing Brain in Living Zebrafish using Brainbow and Time-lapse Confocal Imaging

DOI :

10.3791/60593-v

March 23rd, 2020

March 23rd, 2020

7,941 Views

1Biology Department, Lewis & Clark College

In vivo imaging is a powerful tool that can be used to investigate the cellular mechanisms underlying nervous system development. Here we describe a technique for using time-lapse confocal microscopy to visualize large numbers of multicolor Brainbow-labeled cells in real time within the developing zebrafish nervous system.

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