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Zebrafish Brain Ventricle Injection

DOI :

10.3791/1218-v

April 6th, 2009

April 6th, 2009

20,560 Views

1Whitehead Institute for Biochemical Research, 2MIT - Massachusetts Institute of Technology

After neural tube formation, the neuroepithelium constricts and folds while the tube fills with embryonic cerebrospinal fluid (eCSF) to form the embryonic brain ventricles. We developed this ventricle injection technique to better visualize the fluid filled space in contrast to the neuroepithelial shape in a live embryo.

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Zebrafish

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