Preparing and Mounting Zebrafish for Microinjections
2:22
Injecting the Brain Ventricle
3:08
Imaging
3:43
Determination of Gene Expression Markers
4:44
Zebrafish Locomotive Behavioral Assay
5:34
Results: Lipopolysaccharide-Induced Neuroinflammation to Assess the Levels of Proinflammatory Mediators
7:02
Conclusion
Transcript
Neuroinflammation is a key player in various neurological disorders. Hence, it is of great interest to research and develop alternative in vivo neuroinflammation models to facilitate studies of pathological development. The technique described her
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This protocol demonstrates the microinjection of lipopolysaccharide into the brain ventricular region in a zebrafish larval model to study the resulting neuroinflammatory response and neurotoxicity.