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Identification of protein complexes with quantitative proteomics in S. cerevisiae

13.2K Views

11:12 min

March 4th, 2009

DOI :

10.3791/1225-v

March 4th, 2009


Transcript

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Protein Complexes

Chapters in this video

0:00

Title

0:45

Introduction

1:17

Preparation of the Bait and SILAC Control Strain

2:44

Grinding of Cells

4:05

Pre-clearing Cell Lysate

5:51

Eluting From IgG Beads by TEV Protease Cleavage

6:45

Trichloroacetic Acid (TCA) Precipitation

8:11

EtOH/ Acetate Precipitation

9:00

Sample Analysis by Western Blotting and/ or Silver Staining

9:30

Representative Results/ Outcome

10:15

Conclusion

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