Identification of protein complexes with quantitative proteomics in S. cerevisiae

0:45

Introduction

1:17

Preparation of the Bait and SILAC Control Strain

2:44

Grinding of Cells

4:05

Pre-clearing Cell Lysate

5:51

Eluting From IgG Beads by TEV Protease Cleavage

6:45

Trichloroacetic Acid (TCA) Precipitation

8:11

EtOH/ Acetate Precipitation

9:00

Sample Analysis by Western Blotting and/ or Silver Staining

9:30

Representative Results/ Outcome

10:15

Conclusion