This work was supported by grants to L.A.P. (P.I.):
National Institutes of Health R21 HD50558, awarded 2005-07; National
Institutes of Health R01 HD045822, awarded 2004-09; Dana Foundation Grant,
awarded 2004-06; Canadian Foundation for Innovation ("CFI" grant), awarded
2008-2012; The Ontario Research Fund Grant, awarded 2008-2012.

Part 1: Prior to participant arriving to the lab
<ol>
<li>Ensure that the room is free of extraneous articles that may be distracting to the participant.</li>
<li>Set-up and load experimental protocol on the fNIRS Hitachi ETG-4000 system.</li>
<li>Set-up your experimental paradigm. Experimental paradigms can be programmed with different presentation software, including Eprime, Presentation, Psyscope or a Matlab based psychology toolbox. Here we use Matlab based psychology toolbox. </li>
<li>Timing is key for data analy...

<ol>
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In this study, we demonstrated the use of a novel, non-invasive fNIRS brain imaging technology to investigate human brain function in relation to human cognition and perception. fNIRS brain imaging may represent the future of non-invasive brain imaging, particularly with infant and child populations, that may one day be widely available in research labs, physicians' offices, and in the school systems allowing clinicians to apply basic scientific findings about the brain to their clinical practice.

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		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
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<td>ETG-4000</td>
<td>&nbsp;</td>
<td>Hitachi</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Matlab</td>
<td>&nbsp;</td>
<td>The Mathworks</td>
<td>&nbsp;</td>
<td>Psychology toolbox</td>
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exploring cognitive functions in babies, children &amp; adults with near infrared spectroscopy

An explosion of functional Near Infrared Spectroscopy (fNIRS) studies investigating cortical activation in relation to higher cognitive processes, such as language1,2,3,4,5,6,7,8,9,10, memory11, and attention12 is underway worldwide involving adults, children and infants 3,4,13,14,15,16,17,18,19 with typical and atypical cognition20,21,22. The contemporary challenge of using fNIRS for cognitive neuroscience is to achieve systematic analyses of data such that they are universally interpretable23,24,25,26, and thus may advance important scientific questions about the functional organization and neural systems underlying human higher cognition.
Existing neuroimaging technologies have either less robust temporal or spatial resolution. Event Related Potentials and Magneto Encephalography (ERP and MEG) have excellent temporal resolution, whereas Positron Emission Tomography and functional Magnetic Resonance Imaging (PET and fMRI) have better spatial resolution. Using non-ionizing wavelengths of light in the near-infrared range (700-1000 nm), where oxy-hemoglobin is preferentially absorbed by 680 nm and deoxy-hemoglobin is preferentially absorbed by 830 nm (e.g., indeed, the very wavelengths hardwired into the fNIRS Hitachi ETG-400 system illustrated here), fNIRS is well suited for studies of higher cognition because it has both good temporal resolution (~5s) without the use of radiation and good spatial resolution (~4 cm depth), and does not require participants to be in an enclosed structure27,28. Participants cortical activity can be assessed while comfortably seated in an ordinary chair (adults, children) or even seated in mom s lap (infants). Notably, NIRS is uniquely portable (the size of a desktop computer), virtually silent, and can tolerate a participants subtle movement. This is particularly outstanding for the neural study of human language, which necessarily has as one of its key components the movement of the mouth in speech production or the hands in sign language. 
The way in which the hemodynamic response is localized is by an array of laser emitters and detectors. Emitters emit a known intensity of non-ionizing light while detectors detect the amount reflected back from the cortical surface. The closer together the optodes, the greater the spatial resolution, whereas the further apart the optodes, the greater depth of penetration. For the fNIRS Hitachi ETG-4000 system optimal penetration / resolution the optode array is set to 2cm. 
Our goal is to demonstrate our method of acquiring and analyzing fNIRS data to help standardize the field and enable different fNIRS labs worldwide to have a common background.

Watch this Scientific Journal Video about Exploring Cognitive Functions in Babies, Children & Adults with Near Infrared Spectroscopy at JoVE.com

Exploring Cognitive Functions in Babies, Children &amp; Adults with Near Infrared Spectroscopy

Here we describe a data collection and data analysis method for functional Near Infrared Spectroscopy (fNIRS), a novel non-invasive brain imaging system used in cognitive neuroscience, particularly in studying child brain development. This method provides a universal standard of data acquisition and analysis vital to data interpretation and scientific discovery.

An explosion of functional Near Infrared Spectroscopy (fNIRS) studies investigating cortical activation in relation to higher cognitive processes, such as ...

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20.4K Views.  University of Michigan, Ann Arbor. Here we describe a data collection and data analysis method for functional Near Infrared Spectroscopy (fNIRS), a novel non-invasive brain imaging system used in cognitive neuroscience, particularly in studying child brain development. This method provides a universal standard of data acquisition and analysis vital to data interpretation and scientific discovery.