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Growth Factor-Coated Bead Placement on Dorsal Forebrain Explants

DOI :

10.3791/134-v

January 30th, 2007

January 30th, 2007

7,672 Views

1Department of Developmental and Cell Biology, University of California, Irvine (UCI), 2Department of Physiology and Biophysics, University of California, Irvine (UCI), 3Department of Pathology, University of California, Irvine (UCI)

This video demonstrates two methods for preparing and placing beads, which have been coated with growth factor, on explants of the developing cerebral cortex. These beads can be used to induce spatially restricted gene expression on developing neural tissue such as forebrain explants. Methods are given for using both Affi-Gel beads and heparin acryllic beads.

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Growth Factor coated Bead Placement

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