Growth Factor-Coated Bead Placement on Dorsal Forebrain Explants

DOI :

10.3791/134-v

•

January 30th, 2007

•

7,675 Views

D. Spencer Currle¹, Aaron Kolski-Andreaco², Edwin S. Monuki³

¹Department of Developmental and Cell Biology, University of California, Irvine (UCI), ²Department of Physiology and Biophysics, University of California, Irvine (UCI), ³Department of Pathology, University of California, Irvine (UCI)

Chapters

This video demonstrates two methods for preparing and placing beads, which have been coated with growth factor, on explants of the developing cerebral cortex. These beads can be used to induce spatially restricted gene expression on developing neural tissue such as forebrain explants. Methods are given for using both Affi-Gel beads and heparin acryllic beads.

Related Videos

Mouse Dorsal Forebrain Explant Isolation

Optimized Fibrin Gel Bead Assay for the Study of Angiogenesis

Placing Growth Factor-Coated Beads on Early Stage Chicken Embryos

Preparation and Culture of Rat Lens Epithelial Explants for Studying Terminal Differentiation

Isolation and Purification of Drosophila Peripheral Neurons by Magnetic Bead Sorting

Primary Human Bronchial Epithelial Cells Grown from Explants

Deferred Growth Inhibition Assay to Quantify the Effect of Bacteria-derived Antimicrobials on Competition

Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry

Medium-throughput Screening Assays for Assessment of Effects on Ca²⁺-Signaling and Acrosome Reaction in Human Sperm

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing