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An Approach to Enhance Alignment and Myelination of Dorsal Root Ganglion Neurons

DOI :

10.3791/54085-v

August 24th, 2016

August 24th, 2016

8,146 Views

1Department of Chemical Engineering and Materials Science, Michigan State University, 2Department of Biochemistry and Molecular Biology, Michigan State University

This protocol describes the isolation of dorsal root ganglion (DRG) neurons isolated from rats and the culture of DRG neurons on a static pre-stretched cell culture system to enhance axon alignment, with subsequent co-culture of Schwann Cells (SCs) to promote myelination.

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