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Simple Microfluidic Devices for in vivo Imaging of C. elegans, Drosophila and Zebrafish

DOI :

10.3791/3780-v

September 30th, 2012

September 30th, 2012

16,847 Views

1Neurobiology, NCBS-TIFR, 2Department of Biological Sciences, TIFR

A simple microfluidic device has been developed to perform anesthetic free in vivo imaging of C. elegans, intact Drosophila larvae and zebrafish larvae. The device utilizes a deformable PDMS membrane to immobilize these model organisms in order to perform time lapse imaging of numerous processes such as heart beat, cell division and sub-cellular neuronal transport. We demonstrate the use of this device and show examples of different types of data collected from different model systems.

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Microfluidic Devices

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