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In vivo-like Organotypic Murine Retinal Wholemount Culture

DOI :

10.3791/1634-v

January 11th, 2010

January 11th, 2010

17,593 Views

1Institute for Anatomy, Department of Neuroanatomy, University of Duisburg-Essen

This video article demonstrates the establishment of organotypic retinal wholemount cultures and a cytospin procedure for analysis of exogenously induced effects. Organotypic retinal wholemount cultures mimic the in vivo situation and significantly facilitate the accessibility of murine retinas for experimental manipulations while circumventing the disadvantages of classical murine animal models.

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