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Efficient Dissection and Culture of Primary Mouse Retinal Pigment Epithelial Cells

DOI :

10.3791/62228-v

February 10th, 2021

February 10th, 2021

5,353 Views

1Ocular Genomics Institute of Massachusetts Eye and Ear, Harvard Medical School

This protocol, which was originally reported by Fernandez-Godino et al. in 20161, describes a method to efficiently isolate and culture mouse RPE cells, which form a functional and polarized RPE monolayer within one week on Transwell plates. The procedure takes approximately 3 hours.

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