JoVE Logo
Faculty Resource Center

Sign In

Measurement of Energy Metabolism in Explanted Retinal Tissue Using Extracellular Flux Analysis

DOI :

10.3791/58626-v

January 7th, 2019

January 7th, 2019

9,126 Views

1Division of Metabolism, Endocrinology and Lipid Research, Department of Medicine, Washington University School of Medicine, 2Department of Biomedical Engineering, Washington University in Saint Louis, 3Department of Ophthalmology and Visual Science, Washington University School of Medicine

This technique describes real time recording of oxygen consumption and extracellular acidification rates in explanted mouse retinal tissues using an extracellular flux analyzer.

-- Views

Related Videos

article

Isolation and Culture of Primary Synovial Macrophages and Fibroblasts from Murine Arthritis Tissue

article

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

article

Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

article

Improved Lipofuscin Models and Quantification of Outer Segment Phagocytosis Capacity in Highly Polarized Human Retinal Pigment Epithelial Cultures

article

Direct Synthesis of EM-Visible Gold Nanoparticles in Cells for Protein Localization Analysis with Well-Preserved Ultrastructure

article

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

article

Detection of Mitophagy in Caenorhabditis elegans and Mammalian Cells Using Organelle-Specific Dyes

article

Author Spotlight: Exploring Cellular Zinc Regulation Through ZnT1 Functionality

article

Decellularization-Based Quantification of Skeletal Muscle Fatty Infiltration

article

Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved