February 4th, 2010
•This procedure describes a method for the isolation and culture of the murine organ of Corti with or without the spiral limbus and spiral ganglion neurons. We also demonstrate a method for the expression of an exogenous reporter gene in the organ of Corti explant by electroporation.
Tags
Related Videos
Electroporation of Mycobacteria
The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
Primary Culture of Adult Rat Heart Myocytes
Isolation and Primary Culture of Rat Hepatic Cells
Isolation and Culture of Mouse Primary Pancreatic Acinar Cells
Isolation and Primary Culture of Mouse Aortic Endothelial Cells
Fat Body Organ Culture System in Aedes Aegypti, a Vector of Zika Virus
Novel Method of Plasmid DNA Delivery to Mouse Bladder Urothelium by Electroporation
Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions
Isolation and Culture of Primary Aortic Endothelial Cells from Miniature Pigs
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved