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Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons

DOI :

10.3791/2373-v

15:40 min

January 24th, 2011

January 24th, 2011

32,477 Views

1Department of Anatomy, University of Wisconsin-Madison

This protocol outlines the steps required to dissect, transfect via electroporation and culture mouse hippocampal and cortical neurons. Short-term cultures may be used for studies of axon outgrowth and guidance, while long-term cultures can be used for studies of synaptogenesis and dendritic spine analysis.

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Nucleofection

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