February 16th, 2010
•Protein extraction for proteomic analyses in fungal species requires high levels of standardization to be accomplished according with the minimum information about a proteomic experiment (MIAPE) guidelines. We present a video-protocol that includes a procedure for minimizing experimental bias during toxin induction and protein extraction from Fusarium spp.
Related Videos
Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region
Electrophoretic Separation of Proteins
Interview: Protein Folding and Studies of Neurodegenerative Diseases
Preparation of Rat Brain Aggregate Cultures for Neuron and Glia Development Studies
Digital Microfluidics for Automated Proteomic Processing
Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery
DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses
Sexual Development and Ascospore Discharge in Fusarium graminearum
Naïve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures
Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved