May 3rd, 2013
•We report a method to isolate naïve multipotent skin-derived precursor (SKP) cells from primary human fibroblast cultures. We show that these SKPs derived from fibroblast cultures share similar stem cell properties to the ones derived directly from human skin biopsies. These cells express the neural crest marker, nestin, in addition to the multipotent markers such as OCT4 and Nanog.
Related Videos
Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells (Video) | JoVE
Establishing Primary Adult Fibroblast Cultures From Rodents (Video) | JoVE
Isolation of Stem Cells from Human Pancreatic Cancer Xenografts (Video) | JoVE
Primary Cell Cultures from Drosophila Gastrula Embryos (Video) | JoVE
Isolation and Culture of Adult Epithelial Stem Cells from Human Skin (Video) | JoVE
Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts (Video) | JoVE
Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells (Video) | JoVE
Rapid Fibroblast Removal from High Density Human Embryonic Stem Cell Cultures (Video) | JoVE
Isolation and Culture of Myofibers and their Satellite Cells | Protocol
Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates (Video) | JoVE
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved