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Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures

DOI :

10.3791/50535-v

September 28th, 2013

September 28th, 2013

13,920 Views

1Signaling Proteomics, Max Plank Institute of Molecular Plant Physiology, 2Department of Plant Systems Biology, University of Hohenheim

Here we describe a robust method for the fractionation of plant plasma membranes into detergent resistant and detergent soluble membranes based on a mixture of unlabeled and in vivo fully 15N labeled Arabidopsis thaliana cell cultures. The procedure is applied for comparative proteomic studies to understand signaling processes.

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Metabolic Labeling

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