September 28th, 2013
•Here we describe a robust method for the fractionation of plant plasma membranes into detergent resistant and detergent soluble membranes based on a mixture of unlabeled and in vivo fully 15N labeled Arabidopsis thaliana cell cultures. The procedure is applied for comparative proteomic studies to understand signaling processes.
Tags
Related Videos
Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies
Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression
Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry
Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate
A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
Histochemical Staining of Arabidopsis thaliana Secondary Cell Wall Elements
Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures
Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells
Analysis of Arabidopsis thaliana Growth Behavior in Different Light Qualities
Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved