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Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Utilizing in ovo Electroporation

9.2K Views

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07:55 min

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May 2nd, 2010

DOI :

10.3791/1792-v

May 2nd, 2010

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1Department of Medical Neurobiology, Institute for Medical Research Israel Canada, Hebrew University-Hadassah Medical School

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Axonal Pathways
Genetically Defined Neurons
Chick Neural Tube
In Ovo Electroporation
Enhancer Elements
Reporter Genes
Tracking Axonal Projection
Spinal Interneurons
Germ Line targeted Reporter Genes
Bilateral Labeling
Ipsi And Contralaterally Projecting Axons
Unilateral Electroporation
Central Nervous System
Handling Eggs
Injection
Tungsten Electrodes
Electrical Pulses
Pulse Generator
Incubator
Spinal Cord Preparation
Whole Mount Antibody Staining
Confocal Microscopy

Chapters in this video

0:00

Title

0:11

Introduction

0:50

Egg Handling and Preparing for Electroporation

2:45

Windowing and Electroporation

4:56

Spinal Cord Open-Book Preparation

6:53

Chick Neural Tube in Ovo Electroporation Representative Results

7:25

Conclusion

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