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Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

DOI :

10.3791/1919-v

7:27 min

April 29th, 2010

April 29th, 2010

13,321 Views

1Howard Hughes Medical Institute, Rockefeller University

The fate of the replisome following a collision with a head-on RNA polymerase (RNAP) is unknown. We find that the replisome stalls upon collision with a head-on RNAP, but resumes elongation after displacing the RNAP from DNA. Mfd promotes replication restart by facilitating displacement of the RNAP after the collision.

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Replication Fork

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