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Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

DOI :

10.3791/3255-v

7:18 min

October 27th, 2011

October 27th, 2011

39,100 Views

1Department of Molecular Oncology, Weatherall Institute of Molecular Medicine, University of Oxford , 2Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw

DT40, a model vertebrate genetic system, provides a powerful tool to analyze protein function. Here we describe a simple method that allows qualitative analysis of parameters that influence DNA synthesis during the S-phase in DT40 cells at the single molecule level.

Tags

DNA Replication

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