Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate

1.1K Views

10:32 min

May 19th, 2023

DOI :

10.3791/65413-v

May 19th, 2023


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Vascularized Osteogenic Niche

Chapters in this video

0:00

Introduction

1:04

Establishing the 3D Stromal Monoculture

2:44

Establishing the 3D Stromal‐Endothelial Cell Co‐Culture

4:25

Quantifying the Endothelial Cell Network Formation after the 3D Stromal‐Endothelial Cell Co‐Culture

6:53

Assessing the Osteogenic Differentiation of 3D Stromal‐Endothelial Cells by Monitoring the Alkaline Phosphatase Activity

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