August 1st, 2017
•A method to create and live-image different humanized bone-marrow niches in mice is presented. Based on the supportive niche created by human mesenchymal cells, the addition of human endothelial cells induces the formation of human vessels, while the addition of rhBMP-2 induces the formation of human-mouse chimeric mature bone tissue.
Tags
Related Videos
Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix
Isolating Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow
In Vitro Differentiation of Mature Myofibers for Live Imaging
Live Confocal Imaging of Developing Arabidopsis Flowers
Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry
In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana
Live Imaging of Mouse Secondary Palate Fusion
A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation
Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice
Live Cell Imaging of Chromosome Segregation During Mitosis
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved