Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix

DOI :

10.3791/51999-v

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9:00 min

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February 9th, 2015

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26,968 Views

Nayeli Rodríguez-Fuentes¹, Olivia Reynoso-Ducoing², Ana Rodríguez-Hernández², Javier R. Ambrosio-Hernández², Maria C. Piña-Barba¹, Armando Zepeda-Rodríguez³, Marco A. Cerbón-Cervantes⁴, José Tapia-Ramírez⁵, Luz E. Alcantara-Quintana⁶

¹Depto. Materiales Metálicos y Cerámicos, Instituto de Investigaciones en Materiales, Universidad Nacional Autónoma de México (UNAM), ²Depto. of Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), ³Depto. Biología Celular y Tisular, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), ⁴Depto de Biología Celular, Facultad de Química, Universidad Nacional Autónoma de México (UNAM), ⁵Depto. Genetica y Biología Molecular, Cinvestav-IPN, ⁶Subd. de Investigación, Centro Nacional de la Transfusión Sanguínea, Secretaria de Salud

Chapters

Mesenchymal stem cells (MSCs) have a differentiation potential towards osteoblastic lineage when they are stimulated with soluble factors or specific biomaterials. This work presents a novel option for the delivery of MSCs from human amniotic membrane (AM-hMSCs) that employs the bovine bone matrix Nukbone (NKB) as a scaffold.

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