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Calibration of the Mass Spectrometer for Screening Lipids with Stable Isotope Labeling by LC-TIMS-TOF MS/MS

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02:50 min

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August 23rd, 2024

DOI :

10.3791/201032-v

August 23rd, 2024

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Katherine D. Castro¹, Lilian Valadares Tose¹, Matthew Willetts², Melvin A. Park², Marcela Nouzova³, Fernando G. Noriega⁴^,⁵, Francisco Fernandez-Lima¹^,⁶

¹Deparment of Chemistry and Biochemistry, Florida International University, ²Bruker Daltonics Inc., ³Institute of Parasitology, Biology Centre CAS, ⁴Department of Biological Sciences, Florida International University, ⁵Department of Parasitology, University of South Bohemia, ⁶Biomolecular Sciences Institute, Florida International University

Transcript

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Mass Spectrometer Calibration

LC TIMS TOF MS MS

TIMS Control Software

Calibration Curve

Lipid Internal Standard Mixture

Deuterated Internal Standard

Stable Isotope Labeling SIL

Fatty Acid Chains

Data Analysis Software

Peak Shape Test

Sample List Management

From the series

Screening Lipid Structures Using Stable Isotope Labeling

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