Cultivating Ex Vivo Patient-Derived Glioma Organoids Using a Tissue Chopper

1.6K Views

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05:45 min

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January 19th, 2024

DOI :

10.3791/65952-v

January 19th, 2024

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Marah Alsalkini¹, Veronika Cibulková¹, Maria Breun², Almuth F. Kessler², Tim Schulz², Andrea Cattaneo², Christoph Wipplinger², Julian Hübner³, Ralf-Ingo Ernestus², Thomas Nerreter³, Camelia M. Monoranu⁴, Carsten Hagemann¹, Mario Löhr², Vera Nickl²

¹Section Experimental Neurosurgery, Department of Neurosurgery, University Hospital Würzburg, ²Department of Neurosurgery, University Hospital Würzburg, ³Department of Hematology, University Hospital Würzburg, ⁴Department of Neuropathology, Institute of Pathology, University Hospital Würzburg

Summary

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Ex Vivo Glioma Organoids

Patient derived Organoids

Glioblastoma

Drug Screening

Immunotherapy Screening

Organoid Preparation Methods

3D Organoids

Mechanical Chopping

Automated Chopping Machine

Tumor Characteristics

Manufacturing Time Reduction

Organoid Production Efficiency

Molecular Heterogeneity

IDH wild Type GBM

Chapters in this video

0:00

Introduction

2:25

Processing Tumors with Chopper to Obtain Patient‐Derived Organoids

4:21

Culturing Chopper‐Processed Tumor Pieces for Generating Patient‐Derived 3D Glioma Organoids

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