August 25th, 2022
•Here, we describe a method of generating glioblastoma (GBM) organoids from primary patient specimens or patient-derived cell cultures and maintaining them to maturity. These GBM organoids contain phenotypically diverse cell populations and recreate tumor microenvironments ex vivo.
Related Videos
Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy
Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography
Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay
Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture
Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions
Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds
Ex Vivo Organoid Model of Adenovirus-Cre Mediated Gene Deletions in Mouse Urothelial Cells
Phospholipid Mediator Induced Transformation in Three-Dimensional Cultures
Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved