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Recording Multicellular Behavior in Myxococcus xanthus Biofilms using Time-lapse Microcinematography

DOI :

10.3791/2038-v

August 6th, 2010

August 6th, 2010

12,155 Views

1Department of Environmental Health Sciences, University of South Carolina (USC), 2Department of Biology, Syracuse University

To study Myxococcus xanthus swarm behavior, we have designed a time-lapse microcinematography protocol that can be modified for different assays. It employs standard growth conditions adapted for microscopy, and yields reproducible results by the use of inexpensive, reusable silicone gaskets. We have used this method to quantify multicellular chemotaxis.

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Multicellular Behavior

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