JoVE Logo
Faculty Resource Center

Sign In

Recording Multicellular Behavior in Myxococcus xanthus Biofilms using Time-lapse Microcinematography

DOI :

10.3791/2038-v

August 6th, 2010

August 6th, 2010

12,161 Views

1Department of Environmental Health Sciences, University of South Carolina (USC), 2Department of Biology, Syracuse University

To study Myxococcus xanthus swarm behavior, we have designed a time-lapse microcinematography protocol that can be modified for different assays. It employs standard growth conditions adapted for microscopy, and yields reproducible results by the use of inexpensive, reusable silicone gaskets. We have used this method to quantify multicellular chemotaxis.

Tags

Multicellular Behavior

-- Views

Related Videos

article

Monitoring Actin Disassembly with Time-lapse Microscopy

article

Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos

article

Time-lapse Imaging of Mitosis After siRNA Transfection

article

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans

article

Quantitative Analysis of Random Migration of Cells Using Time-lapse Video Microscopy

article

Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts

article

Use of Time Lapse Microscopy to Visualize Anoxia-induced Suspended Animation in C. elegans Embryos

article

Acquiring Fluorescence Time-lapse Movies of Budding Yeast and Analyzing Single-cell Dynamics using GRAFTS

article

Visualization of Craniofacial Development in the sox10: kaede Transgenic Zebrafish Line Using Time-lapse Confocal Microscopy

article

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved