June 20th, 2010
•A rapid way is described to gain insights into the structure of polysaccharides in an extracellular matrix. The method takes advantage of the specificity of glycosylhydrolases and the sensitivity of mass spectrometry allowing minute amounts of materials to be analyzed. This technique is adaptable to be used directly on tissue itself.
Tags
Related Videos
MALDI Sample Preparation: the Ultra Thin Layer Method
Antibody Profiling by Luciferase Immunoprecipitation Systems (LIPS)
Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS)
Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes
Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry
Glycan Profiling of Plant Cell Wall Polymers using Microarrays
Analysis of Translation Initiation During Stress Conditions by Polysome Profiling
Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis
Cellular Redox Profiling Using High-content Microscopy
TChIP-Seq: Cell-Type-Specific Epigenome Profiling
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved