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OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides

DOI :

10.3791/2046-v

8:43 min

June 20th, 2010

June 20th, 2010

13,303 Views

1Energy Biosciences Institute, University of California, Berkeley, 2Department of Plant and Microbial Biology, University of California, Berkeley

A rapid way is described to gain insights into the structure of polysaccharides in an extracellular matrix. The method takes advantage of the specificity of glycosylhydrolases and the sensitivity of mass spectrometry allowing minute amounts of materials to be analyzed. This technique is adaptable to be used directly on tissue itself.

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OLIgo Mass Profiling

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