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Dechorionation of Medaka Embryos and Cell Transplantation for the Generation of Chimeras

DOI :

10.3791/2055-v

9:03 min

December 22nd, 2010

December 22nd, 2010

17,176 Views

1Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath

Due to the hard chorion and soft embryos, manipulation of medaka embryos is more involved than in zebrafish. This video shows step-by-step procedures for how to manipulate medaka embryos, including dechorionation, mounting in agarose for imaging and cell transplantation for the production of chimeras. These procedures are essential to use medaka and zebrafish in a laboratory to take full advantage of their complementary features for the genetic dissection of vertebrate genome functions.

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Dechorionation

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