Incubate Chick and Quail Eggs to the Desired Stage
1:57
Prepare Eggs for Windowing and Dissection
6:15
Grafting the Tissue
7:21
Immunofluorescent Detection of Quail-specific Nuclear Antigen QCPN in Donor-derived Cells in the Chimeric Embryo
8:35
Conclusion
An approach for analyzing migration and eventual fate of avian neural crest cells in quail-chick chimeric embryos is described. This method is a simple and straightforward technique for tracing neural crest cells during migration and differentiation that are otherwise difficult to distinguish within an unmanipulated chick embryo.