<p class="jove_content">We would like to thank the American Heart Association Mid-Atlantic Affiliate (0555407U,GLB) for funding.</p>

<ol>
	<li>Prior to electrospinning, collagen, from 6 month bovine corium, must be extracted using an acetic acid based process.  This extracted collagen is then purified through a subsequent series of dissolutions, precipitations, and dialyses.  Once collagen is lyophilized, the electrospinning process can begin.</li>
	<li>Three materials are massed: polycaprolactone (PCL), elastin (ELAS), and collagen (COL).  These materials will be separately dissolved in 1,1,1,3,3,3 hexafluoro-2-propanol (HFP, TCI America) at concentrations of 100 mg/ml, 200 mg/ml, and 70 mg/ml, respectively.</li>
	<li>The materials are blended in five different scintillation vials at volumetric ratios of 98-2-0 PCL-ELAS-COL (intima), 45-45-10, 55-35-10, and 65-25-10 PCL-ELAS-COL (media), and 70-0-30 PCL-ELAS-COL (adventitia).</li>
	<li>For uniaxial tensile testing and individual layer compliance testing, each of the materials was individually electrospun, while multi-layered vascular grafts for burst, suture retention, and compliance testing were electrospun from a 3 to 1 configuration.  Uniaxial tensile testing scaffolds were flat and rectangular (2.5 cm wide x 10.2 cm long x 0.3 cm thick) since the 2 mm diameter mandrels used to electrospin individual layers and multi-layered grafts were too small for punching test samples.</li>
	<li>For both individual and multi-layer electrospinning, the polymer solutions were loaded into a plastic 3 ml Becton Dickinson syringe with an 18 gage blunt-tip needle, and placed onto a syringe pump at a set dispensary rate.  Individual electrospinning rates were set to 4 ml/hr, while multi-layered electrospinning rates were varied depending on transition and layer.  Flow rates and volumes were as such:  the intima was electrospun at a rate of 4 ml/hr and a volume of 0.5 ml followed by a transition combining both intimal and medial syringes for 0.2 ml at 2 ml/hr each.  The intimal syringe was then shut off and the medial layer was allowed to spin for 0.6 ml at 4 ml/hr followed by a transition between the media and adventitia for 0.2 ml of polymer solution at 2 ml/hr each.  Finally, the media was stopped and the adventitia was allowed to spin for 0.4 ml at 4 ml/hr.</li>
	<li>All samples were cross-linked in 50 mM EDC for 18 hours prior to any testing <sup>1</sup>.</li>
	<li>Uniaxial tensile testing was performed on six samples from one electrospun sheet hydrated in PBS for 24 hours.  "Dog-bone" shaped samples were punched from electrospun mats and tested on a MTS Bionix 200 testing system with a 100 N load cell and an extension rate of 10.0 mm/min.  Peak stress, modulus, and strain at break were calculated using TestWorks version 4.</li>
	<li>Suture retention testing was performed on six 2 mm inner diameter tubular specimens from six different electrospun grafts, soaked in PBS for 24 hours at 37&deg;C, on a MTS Bionix 200 testing system with a 50 N load cell (MTS Systems Corp.) and an extension rate of 150.0 mm/min in accordance with the straight across procedure described in the American National Standards Institute <sup>2</sup>.  5-0 commercial PDS II violet monofilament suture was placed 2 mm from the end of the sample and extended until the suture had pulled through the graft.  Peak load was recorded in grams-force using TestWorks version 4.</li>
	<li>Burst strength testing was performed on six samples from six different electrospun grafts.  Burst tubes, 2-3 cm in length, were hydrated in PBS, fitted over 1.5 mm diameter nipples attached to the device, and secured with 2-0 silk suture.  Air was introduced into the system, increasing the pressure at a rate of 5 mmHg/s until the tubes burst 2. Results are reported as the pressure in mmHg at which tubes ruptured.</li>
	<li>Dynamic compliance was determined for six 2 mm inner diameter tubular grafts taken from six different electrospun grafts at a length of 3 cm under simulated physiological conditions.  Both individual layers and multi-layered tubular constructs were electrospun and tested under the same conditions.  The specimens were tested in an Intelligent Tissue Engineering via Mechanical Stimulation (ITEMS) Bioreactor developed by Tissue Growth Technologies filled with PBS at 37&deg;C.  The bioreactor provided a 1Hz cyclic pressure change to the inside of the graft, where three different pressure levels of 90/50, 120/80, and 150/110 mmHg systolic/diastolic were investigated <sup>2, 3</sup>.</li>
</ol>
<p class="jove_title">Representative Results</p>
<p class="jove_content">When the electrospinning protocols are carried out correctly, the end product should be a soft, seamless tube with no initial signs of delamination between the layers.  When the uniaxial tensile tests, burst strength tests, suture retention tests, and compliance tests are performed, the results should indicate that as the medial layer stiffness is increased, with decreased amounts of elastin, the associated mechanical properties should demonstrate a stiffer tube.</p>

<ol>
	<li>McClure, M. J., Sell, S. A., Barnes, C. P., Bowen, W. C., Bowlin, G. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cross-linking+Electrospun+Polydioxanone-Soluble+Elastin+Blends:+Material+Characterization.">Cross-linking Electrospun Polydioxanone-Soluble Elastin Blends: Material Characterization.</a> <em style='font-style: italic'>Journal of Engineered Fibers and Fabrics</em>. <b>3</b>, 1-10 (2008).</li><li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> <em style='font-style: italic'>Cardiovascular implants - Vascular graft protheses</em>. <b>VP20</b>, (1994).</li><li>McClure, M. J., Sell, S. A., Simpson, D. G., Walpoth, B. H., Bowlin, G. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+three-layered+electrospun+matrix+to+mimic+native+arterial+architecture+using+polycaprolactone,+elastin,+and+collagen:+A+preliminary+study.">A three-layered electrospun matrix to mimic native arterial architecture using polycaprolactone, elastin, and collagen: A preliminary study.</a> <em style='font-style: italic'>Acta Biomater</em>. , (2010).</li></ol>

<p class="jove_content">Several authors have United States and International patents pending concerning technology presented in this manuscript, and this technology has been licensed to Organogenesis, Inc.</p>

<p class="jove_content">The most critical portion of this study is the electrospinning process.  When using a 3-1 input-output nozzle, arching and charge loss may occur.  If this does occur, the voltage associated with the polymer that is spinning will decrease causing welded, "wet" fibers and creating delamination between each of the three layers.  Therefore, consistent electric potentials are essential to obtain an ideal multi-layered tube.</p>
<p class="jove_content">Compliance mismatch is one of the main causes of graft occlusion at the sm...

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		<div>
		<table border="1">
		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>Polycaprolactone</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Elastin</td>
<td>&nbsp;</td>
<td>Elastin Products Company</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Collagen</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Acetic Acid</td>
<td>&nbsp;</td>
<td>Fisher</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Sodium Chloride</td>
<td>&nbsp;</td>
<td>Fisher</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>TRIS</td>
<td>&nbsp;</td>
<td>Fisher</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>6 L Avanti J-HC Centrifuge</td>
<td>&nbsp;</td>
<td>Beckman Coulter</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>1,1,1,3,3,3 hexafluoro-2-propanol</td>
<td>&nbsp;</td>
<td>TCI America</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>3 ml Becton Dickinson Syringe</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>CZE1000R Rack Mount Power Supply</td>
<td>&nbsp;</td>
<td>Spellman</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>High-Pressure Syringe Pump</td>
<td>&nbsp;</td>
<td>Cole Parmer</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide</td>
<td>&nbsp;</td>
<td>Pierce</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>70 % ethanol</td>
<td>&nbsp;</td>
<td>Sigma Aldrich</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>ImageTool 3.0 software</td>
<td>&nbsp;</td>
<td>Shareware provided by UTHSCSA</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Scanning Electron Microscope</td>
<td>&nbsp;</td>
<td>Zeiss EV050</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>MTS Bionix 200 testing system</td>
<td>&nbsp;</td>
<td>MTS Systems Corp.</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>TestWorks version 4</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Intelligent Tissue Engineering via Mechanical Stimulation (ITEMS) Bioreactor</td>
<td>&nbsp;</td>
<td>Tissue Growth Technologies</td>
<td>&nbsp;</td>
<td>&nbsp;</td>		</tbody>
		</table>
		</div>
	

tri-layered electrospinning to mimic native arterial architecture using polycaprolactone, elastin, and collagen: a preliminary study

<p class="jove_content">Throughout native artery, collagen and elastin play an important role, providing a mechanical backbone, preventing vessel rupture, and promoting recovery under pulsatile deformations.  The goal of this study was to mimic the structure of native artery by fabricating a multi-layered electrospun conduit composed of poly(caprolactone) (PCL) with the addition of elastin and collagen with blends of 45-45-10, 55-35-10, and 65-25-10 PCL-ELAS-COL to demonstrate mechanical properties indicative of native arterial tissue, while remaining conducive to tissue regeneration.  Whole grafts and individual layers were analyzed using uniaxial tensile testing, dynamic compliance, suture retention, and burst strength.  Compliance results revealed that changes to the middle/medial layer changed overall graft behavior with whole graft compliance values ranging from 0.8 - 2.8 % / 100 mmHg, while uniaxial results demonstrated an average modulus range of 2.0 - 11.8 MPa.  Both modulus and compliance data displayed values within the range of native artery.  Mathematical modeling was implemented to show how changes in layer stiffness affect the overall circumferential wall stress, and as a design aid to achieve the best mechanical combination of materials.  Overall, the results indicated that a graft can be designed to mimic a tri-layered structure by altering layer properties.</p>

Watch this Scientific Journal Video about Tri-layered Electrospinning to Mimic Native Arterial Architecture using Polycaprolactone, Elastin, and Collagen: A Preliminary Study at JoVE.com

Tri-layered Electrospinning to Mimic Native Arterial Architecture using Polycaprolactone, Elastin, and Collagen: A Preliminary Study

<p class="jove_content">The aim of this study was to mimic the native three layered architecture of the arterial wall.  To accomplish this, electrospinning was employed with the use of a 3-1 (input-output) nozzle and blends of polycaprolactone, elastin, and collagen.</p>

Throughout native artery, collagen and elastin play an important role, providing a mechanical backbone, preventing vessel rupture, and promoting recovery ...

tri-layered-electrospinning-to-mimic-native-arterial-architecture

Research

JoVE Journal

Bioengineering

12.8K Views.  Virginia Commonwealth University. The aim of this study was to mimic the native three layered architecture of the arterial wall. To accomplish this, electrospinning was employed with the use of a 3-1 (input-output) nozzle and blends of polycaprolactone, elastin, and collagen.