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Isolation of Stem Cells from Human Pancreatic Cancer Xenografts

DOI :

10.3791/2169-v

11:44 min

September 26th, 2010

September 26th, 2010

21,953 Views

1Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine

Cancer stem cells (CSCs) have been identified in a number of malignancies. In this protocol we describe a flow cytometric method utilizing aldehyde dehydrogenase activity and CD44 and CD24 expression to isolate CSCs from human pancreatic adenocarcinoma xenografts. These viable cells can then be used in functional and analytical studies.

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Isolation

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