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07:03 min
October 2nd, 2010
DOI :
10.3791/2185-v
Chapters
0:00
Title
0:50
Introduction
1:08
Growing Plants and Preparing Bacteria
2:58
Leaf Staining and Incubation with Bacteria
4:34
Microscopy, Measurement, and Data Analysis
5:21
Representative Images
6:43
Conclusion
Summary
We have developed a simple and reproducible protocol to access stomatal response to live bacteria. This method minimizes wounding and manipulation of the leaf as compared to the use of epidermal peels reported previously.
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