JoVE Logo

Sign In

A subscription to JoVE is required to view this content.

In Vivo Recording of Intracellular Signals from a Single Neuron

-- views • 1:13 min

Transcript

Begin with a glass micropipette and fill it with an ionic solution.

Attach the prepared micropipette to a holder with a connector wire. The holder is secured in a micromanipulator.

The setup is connected to an intracellular amplifier, which converts ionic signals within the neuron into readable electrical outputs.

Place a reference electrode on the neck muscles of an anesthetized rat with its skull exposed.

Insert the micropipette into the target brain region. This results in a voltage change. Rectify this change using the amplifier.

Remove any moisture at the site and seal the opening with a sealing agent to minimize brain movements.

Gradually lower the micropipette until a significant increase in resistance is observed, indicating contact of the micropipette with the membrane of a neuron.

Use a transient electrical pulse to disrupt the membrane, creating a temporary opening for micropipette penetration.

Record the electrical signals from within the neuron.

article

01:24

In Vivo Recording of Intracellular Signals from a Single Neuron

Related Videos

99 Views

article

07:23

Whole-cell Patch-clamp Recordings in Brain Slices

Related Videos

59.4K Views

article

10:49

In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity

Related Videos

9.5K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved