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Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells

32.1K Views

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12:59 min

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January 19th, 2011

DOI :

10.3791/2314-v

January 19th, 2011

•
1Department of Biology, University of Waterloo

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Optimized
Transfection Strategy
Expression
Electrophysiological Recording
Recombinant Voltage gated Ion Channels
HEK 293T Cells
In Vitro
Cultured Human Embryonic Kidney Cells
Glass Coverslips
Density
Electrophysiological Recording
Contact
Confounding Effects
Plasma Membrane
Whole cell Patch Clamp Recording
Detectable Currents
Noise Levels
Incubation Periods
Membrane Expression
Cell coverslip Adhesion
Membrane Stability
Temperature
Transfected Cells
High Confluency
Ion Channel Protein Expression
Glass Coverslips
Incubation Period
Cell Attachment
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12:59

Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells

32.1K Views

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