JoVE Logo
Faculty Resource Center

Sign In

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

DOI :

10.3791/2189-v

15:28 min

October 1st, 2010

October 1st, 2010

17,092 Views

1Clayton Foundation Laboratories for Peptide Biology, Salk Institute for Biological Studies

We will demonstrate how to study the effect of a single point mutation on the function of an ion channel.

Tags

Mutagenesis

-- Views

Related Videos

article

Patch Clamp Recording of Ion Channels Expressed in Xenopus Oocytes

article

Pulse-chase Analysis of N-linked Sugar Chains from Glycoproteins in Mammalian Cells

article

Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells

article

Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes

article

Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells

article

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection

article

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

article

Analysis of Cell Cycle Position in Mammalian Cells

article

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue

article

Functional Characterization of Na+/H+ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved