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Paired Patch Clamp Recordings from Motor-neuron and Target Skeletal Muscle in Zebrafish

DOI :

10.3791/2351-v

7:29 min

November 20th, 2010

November 20th, 2010

16,671 Views

1Vollum Institute, Oregon Health and Sciences University

Larval zebrafish represent the first vertebrate model system to allow simultaneous patch clamp recording from a spinal motor-neuron and target skeletal muscle. This video demonstrates the microscopic methods used to identify a segmental CaP motor-neuron and target muscle cells as well as the methodologies for recording from each cell type.

Tags

Patch Clamp Recording

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